|Andreas Wolff, C. Leiterer, Andrea Csaki, and Wolfgang Fritzsche
Institute of Photonic Technology (IPHT), P.O.Box 100239, 07702 Jena, Germany
The controlled incorporation of biomolecules into technical setups offers a parallelized and cost effective integration of building blocks or devices in a technological periphery. Due to its unique properties DNA became a potential tool for realization of such a molecular integration by accessing both the micrometer (by directed binding the microelectrodes) and the nanometer world (by sequence specific base pairing). This talk will present results of different immobilization techniques of long DNA (lambda DNA) and of short DNA superstructures (G-wires). Dielectrophoretic trapping allows the defined immobilization of such molecules with a resolution determined by the utilized micro or nano structures. We applied it to long DNA and studied the binding of the molecules into micrometer electrode gaps using in situ fluorescence (single molecule) observation and/or subsequent AFM imaging. We were able to stretch and align DNA in the gaps controlling the aggregation by parameters of the dielectric field. Typical parameters are 2-8 V for 5 min by a frequency of 100 kHz. These results point to the potential importance of dielectophoretic approaches for molecular nanotechnology.