Protein-Sensitive Materials

Kirstin Wenck and Thomas Schrader

University Duisburg-Essen, Department of Chemistry, Universitätstr. 5, 45117 Essen, Germany




In our cooperation within the priority area "Chemical-Biological Hybrid Compounds" (DFG) we develop protein-sensitive materials.[1] Dendrimers, polymers and SAM-forming lipid building blocks are decorated with specific receptor units tailored for all amino acid residues, sugar diols etc. These materials are utilized in sensing applications in solution, in liposomes and in thin films. A colorimetric output often allows naked-eye detection of hormones or proteins. Sulfated sugars such as heparin are also recognized and quantitatively measured by fluorescence enhancement with exquisite sensitivity in the nanomolar range. Finally, new lipid-anchors adorned with bisphosphonate functionalities have been created for the controlled 2D-assembly of Arg-tagged proteins. In combination with similar recognition units based on the His-tag technology (R. Tampe) an orthogonal strategy is being developed which allows to monitor with SPR-related techniques the functional interaction of two proteins immobilized in the 2D lipid layer.