Novel Integrated Biochips for Highly Sensitive Real-Time Analyses of Biomolecules

Ross Rinaldi

National Nanotechnology Laboratory of CNR-INFM, IIT Research Unit, ISUFI, University of Lecce, Via Arnesano 16, 73100 Lecce, Italy




Different nanotechnological strategies have been selected to implement optical biosensing devices, following a bottom-up or top-down approach, depending on the specific application and/or biomolecular functionalities. I present some possible approaches for the implementation of optical biosensors and Lab-on-chip devices exploiting membrane receptors, hybrid systems, colloidal semiconductor nanocrystals (NCs), novel materials and interfaces and different fluorescence sensing schemes, like Fluorescence Resonance Energy Transfer or Metal Enhanced Fluorescence techniques.

In the first part of the talk, I discuss the realization of a solid-state optical biosensor for dopamine and dopaminergic drugs, based on the immobilization of dopamine receptors in a biomimetic membrane tethered to suitable surfaces. Then, I show our approaches, based on polymeric materials and optical/electrical detection schemes, to implement novel integrated biochips for highly sensitive real-time analyses of biomolecules.