Quantum-Dot/Dendrimer Based Functional Nanotubes for Sensitive Studies of DNA Hybridization


Chuan-Liang Feng1, Xinhua Zhong2, Martin Steinhart3, Jean-Pierre Majoral4, and Wolfgang Knoll1

1Max Planck Institute for Polymer Research, Ackermannweg 10, 55128 Mainz, Germany,

2Department of Chemistry, East China University of Science & Technology, 200237 Shanghai (P. R. China),

3Max Planck Institute of Microstructure Physics, Weinberg 2, 06120 Halle, Germany,

4Laboratoire de Chimie de Coordination, Centre National de la Recherche Scientifique, 205 Route de Narbonne, 31077 Toulouse Cedex 4, France

e-mail: fengc@mpip-mainz.mpg.de

URL: http://www.mpip-mainz.mpg.de/groups/knoll

 

A highly sensitive method for detecting oligonucleotide targets has been developed in quantum dot (QD) functionalized nanotubes (NTs) that contain a cascade energy band gap architecture. The design of the functionalized NTs with multi-functionalities and their efficient assembly with different types of QDs was achieved by layer-by-layer (LBL) approach. This allowed for the formation of fluorescence resonance energy transfer (FRET) structures in the NTs resulting in an enhanced fluorescent emission from dye labeled oligonucleotide targets captured by probe DNA through hybridisation. It is demonstrating that this approach results in a significantly enhance of detection of dye labeled oligonucleotide target analysis. It is quite promising to further increase the sensitivity and detecting limits by tuning the QD emission, adding more types of QDs, or adjusting the distance between the QDs and the chromophores.