Synthesis of Modified Nucleosides for Metal Deposition In and Around DNA


Thomas Carell

LMU Munich, Department for Chemistry and Biochemistry, Butenandtstr. 5-13, 81377 Munich, Germany

e-mail: thomas.carell@cup.uni-muenchen.de

URL: http://www.carell.info

 

The Carell laboratory prepares highly modified nucleosides for biochemical[1] and nanomaterials[2] application. In this lecture I will focus at our results obtained in the DNA materials research field[2a-c]. DNA is currently intensively exploited to create nanostructures. In order to functionalize DNA based nanostructures we explore the concept to combine chemical synthesis, "click-chemistry" and molecular biology methods to attach metal ions and metallic metals (M0) to DNA in order to create conductive nanowires. The first approach is based on the concept of metal ion base pairs. Here the canonical base pairs are replaced by synthetic metal ion coordinating units. DNA assembly induces formation of an array of complexing ligands, which capture metal ions and deposite them on top of each other inside the DNA duplex. In the second approach we use DNA purely as a template to finally deposite metallic silver and gold around the DNA duplex. To this end we synthesize modified nucleobases, which can be incorporated into DNA using either solid phase chemistry or PCR. In this way one obtains modified DNA strands containing up to 2000 bp. The introduced modifications allow the selective deposition of metals around the DNA structure using the Tollens reaction and subsequent Ag-development. This procedure allows to create DNA based conductive nanowires.